Composite
Part:BBa_K3447103:Design
Designed by: Yu Ma, Fei Cao Group: iGEM20_Jilin_China (2020-10-26)
Validation of Arabinose response system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1216
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1155
Illegal XhoI site found at 1666 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 990
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal SapI site found at 972
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in the previous iGEM teams (Glasgow 2017) and in GenBank. Besides, this part is a validation of BBa_K3447001