Composite

Part:BBa_K3447103:Design

Designed by: Yu Ma, Fei Cao   Group: iGEM20_Jilin_China   (2020-10-26)


Validation of Arabinose response system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1155
    Illegal XhoI site found at 1666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 990
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal SapI site found at 972


Design Notes

We added some synonymous mutations to avoid part rules.


Source

We found this sequence data in the previous iGEM teams (Glasgow 2017) and in GenBank. Besides, this part is a validation of BBa_K3447001

References